diff --git a/CHANGELOG b/CHANGELOG
index a266a8a49..443f4f010 100644
--- a/CHANGELOG
+++ b/CHANGELOG
@@ -4,6 +4,7 @@
  * Stop reporting a skipped test in `IntegrationTestCase` when there is no legacy test to run
  * Make the `IntegrationTestCase` and `NodeTestCase` test helpers compatible with PHPUnit 11
  * Cast printed expressions to string so values that cannot be converted to a string (arrays, non-`Stringable` objects, ...) report a usable stack trace at the print location
+ * Make the `include()` function return a `Markup` object so an assigned result is not re-escaped when printed
  * Skip the sandbox `__toString` check on arguments whose PHP parameter type cannot implicitly coerce to string
 
 # 3.27.1 (2026-05-30)
diff --git a/doc/functions/include.rst b/doc/functions/include.rst
index 3700018e5..dadac31cd 100644
--- a/doc/functions/include.rst
+++ b/doc/functions/include.rst
@@ -8,6 +8,18 @@ The ``include`` function returns the rendered content of a template:
     {{ include('template.html.twig') }}
     {{ include(some_var) }}
 
+The returned content is a ``\Twig\Markup`` instance, so it is considered safe
+and is not escaped again when you store it in a variable and print it later:
+
+.. code-block:: twig
+
+    {% set body = include('body.html.twig') %}
+    {{ body }} {# rendered as-is, not re-escaped #}
+
+Beware that, like any safe value, it is not re-escaped for the context it ends
+up in, so only embed it in the same context it was rendered for (typically
+HTML).
+
 Included templates have access to the variables of the active context.
 
 If you are using the filesystem loader, the templates are looked for in the
diff --git a/src/Extension/CoreExtension.php b/src/Extension/CoreExtension.php
index 092cd4777..a49f5795e 100644
--- a/src/Extension/CoreExtension.php
+++ b/src/Extension/CoreExtension.php
@@ -1490,9 +1490,11 @@ final class CoreExtension extends AbstractExtension
      * @param bool                         $ignoreMissing Whether to ignore missing templates or not
      * @param bool                         $sandboxed     Whether to sandbox the template or not
      *
+     * @return string|Markup
+     *
      * @internal
      */
-    public static function include(Environment $env, $context, $template, $variables = [], $withContext = true, $ignoreMissing = false, $sandboxed = false): string
+    public static function include(Environment $env, $context, $template, $variables = [], $withContext = true, $ignoreMissing = false, $sandboxed = false)
     {
         $alreadySandboxed = false;
         $sandbox = null;
@@ -1519,7 +1521,9 @@ final class CoreExtension extends AbstractExtension
                 return '';
             }
 
-            return $loaded->render($variables);
+            $rendered = $loaded->render($variables);
+
+            return '' === $rendered ? '' : new Markup($rendered, $env->getCharset());
         } finally {
             if ($isSandboxed && !$alreadySandboxed) {
                 $sandbox->disableSandbox();
diff --git a/tests/Extension/CoreTest.php b/tests/Extension/CoreTest.php
index ac8839369..faada348a 100644
--- a/tests/Extension/CoreTest.php
+++ b/tests/Extension/CoreTest.php
@@ -29,6 +29,7 @@ use Twig\Error\RuntimeError;
 use Twig\Extension\CoreExtension;
 use Twig\Extension\SandboxExtension;
 use Twig\Loader\ArrayLoader;
+use Twig\Markup;
 use Twig\Sandbox\SecurityError;
 use Twig\Sandbox\SecurityPolicy;
 
@@ -417,6 +418,41 @@ class CoreTest extends TestCase
         $twig->render('index');
     }
 
+    public function testSandboxedIncludeResultStaysEscapedWhenAssigned()
+    {
+        $twig = new Environment(new ArrayLoader([
+            'index' => "{% set body = include('included', sandboxed: true) %}[{{ body }}]",
+            'included' => '{{ evil }}',
+        ]), ['autoescape' => 'html']);
+        $twig->addExtension(new SandboxExtension(new SecurityPolicy([], ['escape'], [], [], ['include']), false));
+
+        $this->assertSame('[&lt;script&gt;]', $twig->render('index', ['evil' => '<script>']));
+    }
+
+    public function testIncludeReturnsMarkupForRenderedContent()
+    {
+        $twig = new Environment(new ArrayLoader(['included' => 'content']));
+
+        $result = CoreExtension::include($twig, [], 'included');
+
+        $this->assertInstanceOf(Markup::class, $result);
+        $this->assertSame('content', (string) $result);
+    }
+
+    public function testIncludeReturnsAnEmptyStringForEmptyContent()
+    {
+        $twig = new Environment(new ArrayLoader(['included' => '']));
+
+        $this->assertSame('', CoreExtension::include($twig, [], 'included'));
+    }
+
+    public function testIncludeReturnsAnEmptyStringForIgnoredMissingTemplate()
+    {
+        $twig = new Environment(new ArrayLoader([]));
+
+        $this->assertSame('', CoreExtension::include($twig, [], 'missing', ignoreMissing: true));
+    }
+
     public function testLastModified()
     {
         $this->assertGreaterThan(1000000000, (new CoreExtension())->getLastModified());
diff --git a/tests/Extension/StringLoaderExtensionTest.php b/tests/Extension/StringLoaderExtensionTest.php
index d37b8f263..b49e7bfbb 100644
--- a/tests/Extension/StringLoaderExtensionTest.php
+++ b/tests/Extension/StringLoaderExtensionTest.php
@@ -23,6 +23,6 @@ class StringLoaderExtensionTest extends TestCase
     {
         $twig = new Environment(new ArrayLoader());
         $twig->addExtension(new StringLoaderExtension());
-        $this->assertSame('something', CoreExtension::include($twig, [], StringLoaderExtension::templateFromString($twig, 'something')));
+        $this->assertSame('something', (string) CoreExtension::include($twig, [], StringLoaderExtension::templateFromString($twig, 'something')));
     }
 }
diff --git a/tests/Fixtures/functions/include/assignment_autoescaping.test b/tests/Fixtures/functions/include/assignment_autoescaping.test
new file mode 100644
index 000000000..aae2ad828
--- /dev/null
+++ b/tests/Fixtures/functions/include/assignment_autoescaping.test
@@ -0,0 +1,10 @@
+--TEST--
+"include" function returns Markup so an assigned result is not re-escaped
+--TEMPLATE--
+{% set assigned = include("foo.twig") %}[{{ assigned }}]
+--TEMPLATE(foo.twig)--
+{{- "a & b"|escape -}}
+--DATA--
+return []
+--EXPECT--
+[a &amp; b]